Et Blopjiyslca ~ta Elsevier
نویسندگان
چکیده
Human JL-calpain is activated maximally by 100-200 JLM Ca2+. Both the 80 kDa and 29 kDa subunits of JL-calpain have a EF-hand type calcium-binding domain. It is known that trivalent terbium ion (Tb3+) mimics Ca2+ in many biological systems. We found that Tb3+ alone transiently activated calpain. However, in the presence of Ca2+ , Tb3+ inhibited JL-calpain with an IC5o of about loo JLM. As high as 10 mM Ca2+ did not significantly shift the IC5o of Tb3+. Preincubating JL-calpain by Ca2+ (before Tb3+ and substrate were added) did not diniinish the inhibition by Tb3+. On the other hand, pretreating JL-calpain with Tb3+ produced that Tb3+ has a slow dissociation rate for the calcium-binding sites when compared to Ca2+. Electrophoretic analysis revealed that terbium ion transiently activated JL-calpain followed by the aggregation of the proteinase.
منابع مشابه
Storing information with extractors
We deal with the problem of storing a set of K elements that are taken from a large universe of size N , such that membership in the set can be determined with high probability by looking at just one bit of the representation. Buhrman et al. show an explicit construction with about K2 logN storing bits. We show an explicit construction with about K1+o(1) storing bits, that gets closer to the op...
متن کاملDissociation constants for carbonic acid determined from field measurements
A number of workers have recently shown that the thermodynamic constants for the dissociation of carbonic acid in seawater of Mehrbach et al. are more reliable than measurements made on artificial seawater. These studies have largely been confined to looking at the internal consistency of measurements of total alkalinity (TA), total inorganic carbon dioxide (TCO2) and the fugacity of carbon dio...
متن کاملIntercomparison of techniques to model high temperature effects on CO2 and energy exchange in temperate and boreal coniferous forests
The increased frequency of high atmospheric temperatures (Ta) predicted under current climate change scenarios may adversely impact net ecosystem productivity (NEP) of temperate and boreal conifers. The ability to simulate CO2 and energy exchange under contrasting Ta and vapor pressure deficit (D) is therefore an important attribute in models used to predict climate change impacts on conifer NE...
متن کاملTaurine supplementation improves economy of movement in the cycle test independently of the detrimental effects of ethanol
Taurine (TA) ingestion has been touted as blunting the deleterious effects of ethanol (ET) ingestion on motor performance. This study investigated the effects of ingestion of 0.6 mL·kg-1 of ET, 6 grams of TA, and ethanol in combination with taurine (ET+TA) on economy of movement (EM) and heart rate (HR). Nine volunteers, five female (22 ± 3 years) and four male (26 ± 5 years), participated in a...
متن کاملAcute effect of Ethanol and Taurine on frontal cortex absolute beta power before and after exercise
Ethanol (ET) is a substance that modulates the Central Nervous System (CNS). Frequently, ET intake occurs combined with energy drinks, which contain taurine (TA), an important amino acid found in the body (i.e brain and muscles). Although TA administration has been used in the improvement of physical performance, the impact of TA, ET and exercise remains unknown. This study aimed to analyze the...
متن کامل